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Course topics: Seminars: 1) Introduction to Mycology 2) History of the study of microscopic fungi and their distribution 3) Isolation, transport, storage and safety principles when working with microscopic fungi. Fundamentals of systematics 4) Reproduction of lower and higher microscopic fungi 5) Morphology and formation of fructifying organs of yeasts 6) Morphology and formation of fructifying organs of microscopic filamentous fungi 7) Dermatophytes. Fundamentals of medical mycology 8) Virulence and pathogenicity factors of candida 9) Distribution of antifungal agents and mechanisms of resistance 10) Diagnostic methods used in the identification and characterization of microscopic filamentous fungi and pathogenic yeasts 11) Diagnostic methods used in the identification and characterisation of microscopic filamentous fungi and pathogenic yeasts 12) Diagnostic methods used in the identification and characterisation of microscopic filamentous fungi and pathogenic yeasts Practise: 1) Introduction to Mycology exercises, general information, credit requirements, study materials. Introduction to safety principles in the biology laboratory. Principles of work organization in the laboratory. 2) Basic methods and equipment used in the cultivation of microscopic filamentous fungi and yeasts. Preparation of soils and inoculation of candida on Sabouraud Dextrose agar and CHROMagar Candida. 3) Evaluation of growth on individual soils. 4) Differentiation of Candida albicans from \"non-C. albicans Candida\" species using Germ Tube test and using biochemical tests API 20 C aux system, reading of results and their correct interpretation - I. 5) Differentiation of Candida albicans from \"non-C. albicans Candida\" species using Germ Tube test and using API 20 C aux system biochemical tests, reading of results and their correct interpretation. 6) Differentiation of Candida albicans from \"non-C. albicans Candida\" species using Germ Tube test and using API 20 C aux system biochemical tests, reading of results and their correct interpretation. 7) Determination of susceptibility/resistance of candida to antifungal agents - azole (fluconazole) and echinocandin (caspofungin) by microdilution method, preparation of 96-well polystyrene plate, reading of results and their correct interpretation - I. 8) Determination of susceptibility/resistance of candida to antifungal agents - azole (fluconazole) and echinocandin (caspofungin) by microdilution method, preparation of 96-well polystyrene plate, reading of results and their correct interpretation. 9) Determination of susceptibility/resistance of candida to antifungal agents - azole (fluconazole) and echinocandin (caspofungin) by microdilution method, preparation of 96-well polystyrene plate, reading of results and their correct interpretation - III. 10) Adherence ability and biofilm formation of candida on 96-well polystyrene plates, quantification using crystal violet spectrophotometrically, graphical evaluation of results - I. 11) Adherence ability and biofilm formation of candida on 96-well polystyrene plates, quantification using crystal violet spectrophotometrically, graphical evaluation of results. - II. 12) Adherence ability and biofilm formation of candida on 96-well polystyrene plates, quantification using crystal violet spectrophotometrically, graphical evaluation of results - III.

Supervisors and educators:
doc. PhDr. Silvia Puteková, PhD. - Kogarant
doc. Ing. Stanislava Blažíčková, PhD. - Zabezpečuje, Hlavný garant
RNDr. Karolína Tomčíková, PhD. - Schvaľujúci zápis
doc. RNDr. Martina Horváthová, PhD. - Kogarant
doc. RNDr. Soňa Kucharíková, PhD. - Zabezpečuje, Skúšajúci, Zodpovedný za elearning, Vedúci semináru, Cvičiaci
PhDr. Michal Rafajdus, PhD. - Kogarant

Code: LMbEE26

Period: ZS

Credits: 2